V-ATPase | epsilon subunit of tonoplast H+ATPase, Blocking peptide
Estimation of the ratio between antibodies and a peptide : In neutralisation assay use 8,13 l of V-ATPase peptide for 10 ml of reaction volume. In case of any questions please contact support@agrisera
Donkey normal serum (2 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
Sheep purified IgG (10 mg)
Sheep IgG is provided in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2. 0.05 % sodium azide is added as preservative.