Agrisera ECL kit (Bright/SuperBright)
This set combines reagents with low pico to mid femtogram and extreme low femtogram detection range combined with low background and superior signal to noise ratios.
AgriseraTMB HRP Substrate
One component ready to use reagent. It contains 2.5 mMol L-1 TMB and Hydrogen Peroxide in a buffer at pH 3.6. It also contains non-toxic stabilizers.
Goat anti-Rabbit IgG (unabsorbed)
Antibody purity is > 95% based on SDS-PAGE.Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v) with sodium Azide is added as a preservative.Based on IEP, this a
AgriseraECL Bright
User Instruction Store reagents A and B in the darkness at 4-8°C.Mix equal volumes of reagent A and B (chemiluminescent substrate) in a clean container and equlibrate to room temperature 30 minutes b